Choose from a three- (M3), four- (M4), or We would like to show you a description here but the site won’t allow us. 9000-0756. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Large Molecule Bioanalysis. Learn how to easily set up an efficient and informative plate read by using SoftMax Pro 6 Microplate Data Acquisition and Analysis software. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. In this study, changes in soil physicochemical properties, as well as bacterial diversity and community structures (by high-throughput. In the M2, M5 and Plus 384, the light. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2 only). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Five hours prior to when permeability will be measured, gavage mice with 0. SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). Note: In this user guide, SpectraMax ® refers to several SpectraMax . Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Western blot apparatus (BIO-RAD Laboratories). How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Large Molecule Bioanalysis. FlexStation ®, SpectraMax. book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. D 47 7. 次いで、ユーザーのラボに. Ltd. Table 1. Detection modalities are shown in Table 1-1. SpectraMax Gemini XPS/EM Register or Sign In. Roelli1, Amandine Stoss1, Rebecca A. 5. The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. The activity of untreated cells was normalized to 100% and the treated cell activity was compared with that. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. STAKMAX. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. com. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The absorbance was measured spectrophotometrically using a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) at 570 nm. We therefore gathered salient information about many of the. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Host your own live radio show with. Multiplate reader for ELISA detection (Spectramax M4). ). B07F4876MS. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M5e reader offers the additional benefit of being certified . Dettmer4, Wayne A. Devices SpectraMax® M4). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. Using. Because of possible losses from settling, particle data for the >7 µm size fraction (<0. Cell viability was calculated using the formula as follows: (OD450 KO HepG2 -OD450 blank /OD450 WT HepG2 -OD450 blank ) × 100%. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Louis, MO, USA). Background-subtracted data values (mean +/- SD) are graphed. instruments you must first insert an adapter plate. 2. Images were visualized using a fluorescence microscope (Nikon, Japan) . However, whether the Rhizoma drynariae total flavonoids (RDTF) can promote the absorption of calcium and enhance the bone formation is unclear. 6 mm). The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Italic and boldface type are used for emphasis. org) with optimized instrument settings (Table 1) were used to generate and analyze data. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 8 0112-0115G CAUTION!Useoforganicsolventscancauseharmtotheopticsintheinstrument. Lysosomal proteolytic activity. 304 signal level saturation. Molecular Devices SpectraMax M4 User Manual (86 pages) Multi-Mode Microplate Readers. Highly selective for dsDNA over ssDNA or RNA. Environmental Chamber and Respiratory SimulatorsRhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. 7°C (gradient heating design). 2. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. 1, BIO-RAD Laboratories). Flasks containing 100 mL of YM broth were inoculated with 100. FlexStation™ I. 313 reference gain check fail. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax Microplate Readers. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. Results and Discussion Small-molecule agonists have been identified for TLR7 [ 41 , 42 , 47 , 48 , 60 – 67 ], TLR8 [ 49 , 51 – 54 , 68 ], TLR4 [ 69 – 72 ] and TLR2 [ 73 , 74 ]. All of the experiments were performed in triplicate, and the results were represented as mean. 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. 1. We would like to show you a description here but the site won’t allow us. All configurations. ). . The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). 6. Background-subtracted data values (mean +/- SD) are graphed. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. TSQ was dissolved in Lock’s buffer (pH 7. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). SigmaPlot (Systat Software, Inc. 1 mg·mL −1 (Sigma-Aldrich, USA) as described above and fluorescence intensity at λ ex = 530 nm and λ em = 590 nm was detected through a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA). /600 em. Category 2 and 3 microplate readers use gradient heating as well. 315 can't find zero order. Following digestion, 200 µl from each sample was pipetted into a 96-well plate, and samples were measured in duplicate at 485 and 525 nm excitation and emission, respectively (SpectraMax M4, Molecular Devices). 3. Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. 50 μl of samples were added into the mixture of 40 μl of detection buffer and 10 μl of Ac-DEVD-pNA (2 mM) with gentle shaking for 5 min. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. , San Jose, CA) was. . GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. . 5 mg mL −1 with 0. 2. 4) In vivo pharmacokinetic studies. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 4. 4) and cells were stained with 150 nM TSQ for 1 min. Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M4 Multi-Mode Microplate Reader. Instruments Ltd. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Lift up the lid over the chamber to insert or remove a cuvette. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Flasks containing 100 mL of YM broth were inoculated with 100. Following incubation, the media was aspirated completely and MTT formazan crystals formed were dissolved by the addition of dimethylsulfoxide, and the reading was taken at 570 nm using SpectraMax M4. Cell uptake studies. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. We have two NanoDrop micro-scale spectrophotometers, a Molecular Devices Spectramax M4 micro plate reader and a UVP gel documentation camera. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). 7°C and the bottom to 36. All configurations. Figure 1. , contact Molecular. 2 pM 2 pM 384-well (50 µL) 3 pM 4 pMCell viability was determined by addition of MTT ([3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma) to cells and incubation for 4 h. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forSpectraMax® M3. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . Cet appareil ISM est confomre à la norme NMB-001 du Canada. M4 Contracting Ltd, Victoria, British Columbia. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Multi-Mode Microplate Readers. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The ability of the liposome to be internalized into cell was assessed using cell culture studies 45-47. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. Description 1. We therefore gathered salient. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. D 1 1. New PBS 1X was added into each well and oxidized DCFH excitation and emission were measured at 485 and 525 nm respectively in a spectrophotometer (SpectraMax M4, Molecular Devices, Sunnyvale, USA). Choose from a three- (M3), four- (M4), or Cables. 2. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). All experiments. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. This platform allows the. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Optical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Graph pad prism 8. Pentobarbital sodium and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Establishment of mouse orthotopic xenograft bladder tumor model. Acridine orange (AO) staining evaluates the morphological changes in apoptotic cells by formation of blebs in the membrane, fragmentation and condensation of the. 3. 306 plate air cal fail,low light. Fastenal is the largest fastener distributor in North America. Choose from a three- (M3), four- (M4), orCables. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Transfer plate to SpectraMax M5 with excitation monochromator set to 455 nm with 10 nm bandwidth, emission monochromator set to 525 nm with 10 nm bandwidth, and 515 nm cutoff filter. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The DQ Green BSA probe. Dumont3, Matthias S. 8. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Background-subtracted data values (mean +/- SD) are graphed. Exhibit with us {{>currencyLabel}} Back. org) with optimized instrument settings (Table 1) were used to generate and analyze data. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] number of moles of FITC conjugated to OVA was determined using UV–vis spectroscopy (SpectraMax M4, Molecular Devices Inc. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. Background-subtracted data values (mean +/- SD) are graphed. Preconfigured SoftMax ® Pro Software protocols (available at softmaxpro. Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. Plot the fluorescence intensity versus the cell seeding number. Dumont3, Matthias S. For labeling representative cells, they were treated under the same conditions as above, followed by fixation with 2% paraformaldehyde for 20 min at room temperature. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. The MTT reagent was incubated with cells for 4 h. Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Add To My List; Create New List; Quickview. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 2. In. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. . Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. High tolerance for contaminants. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. 7% of the total test aerosol mass) was not included in the collection analysis. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). We have 2 Molecular Devices SpectraMax M4 manuals available for free PDF download: User Manual . 314 low lamp level warning. (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities for Detection Estimates, Magnitude 1 through 6. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. 5 pM 2. ZERO BIAS - scores, article reviews, protocol conditions and more7. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. VIEW DETAILS. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Description 1. 0 compensates for most changes in environmental temperature or pressure. , USA). Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. STAKMAX. Polybead® nanoparticles were analyzed. The instrument reads all Plate sections in the experiment. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 6: bioNova científica, s. Eos - M4/3 lens adapter - Lenses - Victoria, British Columbia | Facebook. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. , and red fluorescence at 543 ex. Microscopic evaluation of apoptosis. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 This document is provided to customers who have purchased Molecular Devices,…spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485nm and emissionλ 535nm. SpectraMax M3, M4, M5, and M5 e. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. MTT values at day 3 were normalized to day 0. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). After incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). The pathophysiology of such. The chamber contains springs that position the cuvette in the proper alignment for a read. Spectramax M Series, supplied by Danaher Inc, used in various techniques. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Table of Contents. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. MTT assay was utilized to detect the cytotoxicity of different compounds on HUVECs. Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. Background-subtracted data values (mean +/- SD) are graphed. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 1 & 7. ® reader to the computer. . My List. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. * BD Falcon® Microtest™ plates #353075 and # 353072). Interpolated concentrations of native CRP in human serum and plasma samples. Click Read. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. For heme titration experiments a constant ATP concentration of 1 mM was used. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Plate chamber design of the SpectraMax category 1 microplate readers. Serving Victoria's renovation and construction needs. Standard features include a cuvette port,. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Description 1. When a feature or capability applies to only certain readers, this exception is noted. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). Images were taken using a fluorescence microscope (Nikon 80i, Japan). SpectraMax® L (software version 5. . SPECTROMAXx with iCAL 2. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 2. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. Table 2. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. Statistical analysis. 1. Example: [Drawer]. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 7. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Supplier: Molecular Devices. Real time thermal cyclers (iCycleriQ apparatus with iCyclerOptical System software version 3. The SpectraMax M2 and M2e are versatile and reliable. For more detailed information and technical support of Molecular Devices instruments or software,The sample solution was added to DPPH. Colony Formation Assay. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Expand the ABS1 Plate Inserted or Expt#3 experiment and select the OptAlign&UltDrk Plate section. The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. SpectraMax® M4). DNA standards. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. Detection cartridge modularity allows for easy addition of new read modes within minutes without. e. FlexStation ®, SpectraMax. Laboratory Freezers. 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. August 16, 2017. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Recertification is recommended every two years. Home; Lighting; Work lights; User manual. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. 4. 73. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). Mitochondrial respiration using high-resolution respirometry . C. Plot the fluorescence intensity versus the cell seeding number. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. SpectraMax® Paradigm® Multi-Mode Detection Platform. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells.